anti glut4 rabbit polyclonal ab (Santa Cruz Biotechnology)
Structured Review

Anti Glut4 Rabbit Polyclonal Ab, supplied by Santa Cruz Biotechnology, used in various techniques. Bioz Stars score: 95/100, based on 604 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/anti+glut4+rabbit+polyclonal+ab/Glut4/pm37308077-83-44-69
Average 95 stars, based on 604 article reviews
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1) Product Images from "Pancreatic beta-cell specific BAG3 knockout results in chronic hyperinsulinemia inducing insulin resistance."
Article Title: Pancreatic beta-cell specific BAG3 knockout results in chronic hyperinsulinemia inducing insulin resistance.
Journal: Molecular metabolism
doi: 10.1016/j.molmet.2023.101752
Figure Legend Snippet: Figure 5: BAG3bKO mice displayed peripheral but not hepatic insulin resistance. (A) Venn diagram and volcano plot showing the number of proteins and DEPs detected in muscles of 64-weeks old control and BAG3bKO mice. Down-regulated and up-regulated DEPs identified by MS/MS analysis are highlighted in blue and red, respectively. (B) Significantly inhibited upstream predicted by IPA analysis. (C, D) Representative Western blot of muscle INSR, GLUT4 phospho-AKT/AKT, and phospho-ERK1/2/ERK1/2 expression in control and BAG3bKO mice. Relative protein expression data are presented as mean values SEM; n ¼ 7e8 for each group; *p < 0.05.
Techniques Used: Muscles, Control, Tandem Mass Spectroscopy, Western Blot, Expressing
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Incubation:Article Title: Pancreatic beta-cell specific BAG3 knockout results in chronic hyperinsulinemia inducing insulin resistance. Article Snippet: Equal amounts of proteins were separated on an SDS-PAGE and detected after being transferred to a nitrocellulose membrane (GE Healthcare Life Science). .. The membranes were incubated overnight at 4 C with primary antibodies: anti-BAG3 used 1:2000 (Novus Biologicals, Milan, Italy), anti-INSR used 1:1000, anti-AKT used 1:1000, anti-phospho-AKT used 1:1000, anti-ERK1/2 used 1:1000, antiphospho-ERK1/2 and anti-GAPDH used 1:2000, all supplied by Cell Signaling Technology (Danvers, MA, USA), |